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微信小章| 产品名称： | IEC-6 IEC6 |
|---|---|
| 商品货号： | TS211780 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | small intestine/epithelium |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Comments： | Cell growth is inhibited by cortisol. The cells express antigens characteristic of intestinal epithelia. The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
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| Subculturing： | Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | A Quaroni |
| Deposited As： | Rattus sp. |
| Passage History： | The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed. |
| References： | Quaroni A, et al. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc. Natl. Acad. Sci. USA 75: 5548-5552, 1978. PubMed: 103096 Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453 Quaroni A, et al. Keratin expression in rat intestinal crypt and villus cells. J. Biol. Chem. 266: 11923-11931, 1991. PubMed: 1711043 Dignass AU, Podolsky DK. Interleukin 2 modulates intestinal epithelial cell function in vitro. Exp. Cell Res. 225: 422-429, 1996. PubMed: 8660931 Weiser MM, Quaroni A. A vitamin D-related inhibition of growth of an epithelioid cell line derived from rat small intestine. Biochem. Biophys. Res. Commun. 90: 788-793, 1979. PubMed: 508345 Jakobs ES, et al. Expression of sodium-linked nucleoside transport activity in monolayer cultures of IEC-6 intestinal epithelial cells. J. Biol. Chem. 265: 22210-22216, 1990. PubMed: 2266122 Conteas CN, Majumdar AP. The effects of gastrin, epidermal growth factor, and somatostatin on DNA synthesis in a small intestinal crypt cell line (IEC-6). Proc. Soc. Exp. Biol. Med. 184: 307-311, 1987. PubMed: 2881310 |
| Cross References： | Nucleotide (GenBank) : Z46374 R.norvegicus mRNA for c-met. Nucleotide (GenBank) : AF008587 Rattus norvegicus hereditary hemochromatosis protein homolog (RT1-CAFe) mRNA, partial cds. |