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| 产品名称： | Ig(1a)8.3 (formerly 20-8.3) |
|---|---|
| 商品货号： | TS211782 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a |
| Comments： | Animals were immunized with antigen - antibody complexes (BALB/c anti Bordetella pertussis with B pertussis). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | LA Herzenberg |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Oi VT, Herzenberg LA. Localization of murine Ig-1b and Ig-1a (IgG2a) allotypic determinants detected with monoclonal antibodies. Mol. Immunol. 16: 1005-1017, 1979. PubMed: 94425 Oi VT, et al. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115-129, 1978. PubMed: 567555 |