微信小陈
微信小章| 产品名称： | IgG-11H4 |
|---|---|
| 商品货号： | TS211786 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | Immunoglobulin; monoclonal antibody; against the carboxy terminus of lipoprotein receptor related protein (LRP) |
| Cellular Products： | Immunoglobulin; monoclonal antibody; against the carboxy terminus of lipoprotein receptor related protein (LRP) |
| Comments： | Animals were immunized with a synthetic peptide (corresponding to the 13 carboxy terminal peptides of human LRP) coupled to KLH. The antibody reacts with the carboxy terminus of LRP from various species. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2xa0x 105 viable cells/mL. Maintain cell density between 1xa0x 105 and 1 x 106 viable cells/mL. Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | YK Ho, JL Goldstein |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kowal RC, et al. Low density lipoprotein receptor-related protein mediates uptake of cholesteryl esters derived from apoprotein E-enriched lipoproteins. Proc. Natl. Acad. Sci. USA 86: 5810-5814, 1989. PubMed: 2762297 Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029 |