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微信小章| 产品名称： | IgG-1B3 anti LRP 85 |
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| 商品货号： | TS211787 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts specifically with the 85 kDa subunit of rabbit LRP. It cross-reacts weakly with the 85 kDa subunit of rat LRP. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the 85000 dalton (85 kDa) subunit of rabbit lipoprotein receptor related protein (LRP) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the 85000 dalton (85 kDa) subunit of rabbit lipoprotein receptor related protein (LRP) |
| Comments： | Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 85 kDa subunit of rabbit LRP. It cross-reacts weakly with the 85 kDa subunit of rat LRP. The antibody does not react with the 515 kDa subunit of rabbit LRP. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | YK Ho, JL Goldstein |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |