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| 产品名称： | I1-Hybridoma |
|---|---|
| 商品货号： | TS211788 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Vesicular Stomatitis |
| Age： | adult |
| Applications： | The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Tumorigenic： | Yes |
| Effects： | Yes, intraperitoneal injection into BALB/c or (BALB/c X BALB.B)F1 mice |
| Comments： | Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.). Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.). |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2.0 mM L-glutamine and Hanks BSS adjusted to contain 0.35 g/L sodium bicarbonate 80%; fetal bovine serum, 20%.
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Note: This medium is formulated for use in a free gas exchange with atmospheric air. A 5% CO2 in air atmosphere is detrimental to cells when using this medium for culturing. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 100% |
| Isotype： | mouse IgG2a kappa |
| Name of Depositor： | IS Novella, DS Lyles |
| Deposited As： | Mus musculus (B cell); Mus musculus (myeloma) |
| References： | Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550 |