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微信小章| 产品名称： | ICR 134 (RPH67.134c2) Diploid Frog |
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| 商品货号： | TS211791 |
| Organism： | Rana pipiens, frog, grass |
| Tissue： | gynogenetic haploid embryo |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near diploid |
| Derivation： | This line was derived from the minced tissues of stage 17 gynogenetic haploid embryos of the Vermont population of Rana pipiens. |
| Virus Resistance： | poliovirus; vesicular stomatitis (Indiana); herpes simplex; vaccinia |
| Comments： | The line was cloned at the 11th passage and the cloned line (RPH67.134c2) proved to be near diploid. For haploid cells, see ICR 2A (ATCC CCL-145). |
| Complete Growth Medium： | Leibovitzs L-15 medium, 50%; distilled water, 40%; fetal bovine serum, 10%. Incubate at 25C in air without CO2. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C Atmosphere: Air atmosphere |
| Name of Depositor： | J Freed, L Mezger-Freed |
| Deposited As： | Rana pipiens |
| References： | . Biology of amphibian tumors. New York: Springer-Verlag; 1969. . . Methods Cell Physiol. 4: 20-46, 1970. Mizell, M.; Biology of amphibian tumors. New York: Springer-Verlag; 1969. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |