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微信小章| 产品名称： | IA-XsSBR |
|---|---|
| 商品货号： | TS211793 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | small intestine |
| Cell Type： | Epithelial,Epithelial-like |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma; radiation (X-ray) induced |
| Age： | adult |
| Gender： | male |
| Strain： | outbred Holtzman |
| Applications： | The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat. The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat.
The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene. |
| Clinical Data： | The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat. male |
| Comments： | The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat.
The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | RH Stevens |
| Deposited As： | Rattus sp. |
| References： | Stevens RH, et al. Lymphocyte cytotoxicity in X-irradiation-induced adenocarcinoma of the rat small bowel. I. Measurement of target cell destruction by release of radioiodinated membrane proteins: brief communication. J. Natl. Cancer Inst. 59: 1315-1319, 1977. PubMed: 904002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |