微信小陈
微信小章| 产品名称： | IEC-18 |
|---|---|
| 商品货号： | TS211796 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | ileum |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | IEC-18 was derived from epithelial cells from rat small intestine. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | A Quaroni |
| Deposited As： | Rattus sp. |
| References： | Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453 Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzoapyrene and 7,12- dimethylbenzaanthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638 |
| Cross References： | Nucleotide (GenBank) : S70238 type I interleukin-1 receptor rats, intestinal epithelial IEC-18 cells, ATCC 1589, mRNA Partial, 509 nt. |