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微信小章| 产品名称： | I-13.35 |
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| 商品货号： | TS211797 |
| Organism： | Mus musculus, mouse |
| Tissue： | spleen |
| Cell Type： | macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Strain： | C3H/HeJ |
| Applications： | The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. xa0 |
| Antigen Expression： | CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) + Ref   Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081 |
| Receptor Expression： | colony stimulating factor 1 (CSF-1R, CD115) |
| Comments： | The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor.xa0RefWilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081
This cell line, unlike I-11.15 (ATCCxa0CRL-2470), has the constitutive ability to present antigen to Th1 helper T cells and it expresses higher levels of MHC Class II antigens than I-11-15.xa0Ref C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended LADMAC Conditioned Medium
LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420).xa0 LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1xa0Ref Subculturing Procedure for LADMAC cells Cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Attached cells may be dispersed by tapping the sides of the flask until cells are loose.
Complete Growth Medium for LADMAC cells
The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003.xa0 To make the complete growth medium, add the following components to the base medium:xa0 fetal bovine serum to a final concentration of 10%
This medium is formulated for use with a 5% CO2 in air atmosphere.
Conditioned medium harvesting:
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| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | WS Walker |
| Deposited As： | mouse |
| Passage History： | Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. |
| References： | Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081 |