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微信小章| 产品名称： | I 9.2 |
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| 商品货号： | TS211798 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | This cell line can be used to study the role of caspase-8 in apoptotic signaling pathways in the absence of overexpression. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The I 9.2 cell line is a caspase-8 mutant of the wild-type Jurkat cell line A3 (see ATCC CRL-2570). Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.xa0Ref ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks. Ref |
| Comments： | The I 9.2 cell line is functionally defective for caspase-8 and the I 2.1 cell line is functionally defective for FADD.
The I 9.2 cell line and the I 2.1 cell lines are completely resistant to Fas-induced apoptosis.xa0Ref Complementation of I 9.2 cell line with wild-type caspase-8 restored Fas-mediated apoptosis. Ref The I 9.2 cell line is severely deficient in cell death induced by TNF-alpha and is partially deficient in cell death induced by ultraviolet irradiation, adriamycin and etoposide. Ref
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 2 x 105 and 2 x 106 viable cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 8,11 D16S539: 11 D5S818: 9 D7S820: 8,9.2 THO1: 6,9.3 TPOX: 8,10 vWA: 18 |
| Name of Depositor： | J Blenis |
| Deposited As： | human |
| References： | Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801 Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904 Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182 Ding HF, et al. Essential role for caspase-8 in transcription-independent apoptosis triggered by p53. J. Biol. Chem. 275: 38905-38911, 2000. PubMed: 10988287 |
Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801