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| 产品名称： | I/24.D6 |
|---|---|
| 商品货号： | TS211804 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an epitope encoded by variable exon C of mouse CD45. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD45RC |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD45RC |
| Comments： | Animals were immunized with activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an epitope encoded by variable exon C of mouse CD45. This epitope is expressed at high levels on essentially all mouse B cells and at lower levels on virtually all CD8 positive (CD8+) splenic T cells. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate, 1% non-essential amino acids and 50 uM 2-mercaptoethanol, 80%; ORIGEN (Hybridoma Cloning Factor), 10%; heat-inactivated fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgM |
| Name of Depositor： | G Lazlo, KS Hathcock |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Hathcock KS, et al. Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J. Immunol. 148: 19-28, 1992. PubMed: 1370168 |