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微信小章| 产品名称： | I-11.15 |
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| 商品货号： | TS211807 |
| Organism： | Mus musculus, mouse |
| Cell Type： | macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Strain： | C3H/HeJ |
| Applications： | This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. This cell line, unlike I-13.35 (ATCC CRL-2471), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35. The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) |
| Derivation： | This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. |
| Clinical Data： | female |
| Antigen Expression： | CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) + Ref   Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081 |
| Receptor Expression： | colony stimulating factor 1 (CSF-1R, CD115) |
| Genes Expressed： | CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) + |
| Comments： | This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. The cell line is dependent on colony stimulating factor 1 (CSF-1). The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. This cell line, unlike I-13.35 (ATCC CRL-2471), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35. The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove 75% of the media. Scrape off the attached cells and transfer to new culture flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | WS Walker |
| Deposited As： | mouse |
| References： | Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081 McCormack JM, et al. Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell. Immunol. 145: 359-371, 1992. PubMed: 1451184 |