微信小陈
微信小章| 产品名称： | HT-1376 |
|---|---|
| 商品货号： | TS211808 |
| Organism： | Homo sapiens, human |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade 3, carcinoma |
| Age： | 58 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal chromosome number = 104 to 121 |
| Clinical Data： | 58 years Caucasian female |
| Genes Expressed： | fibrinolytic activity; interferon |
| Cellular Products： | fibrinolytic activity; interferon |
| Tumorigenic： | Yes |
| Effects： | Yes, in mice and hamsters |
| Comments： | The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens. The cells will grow in soft agar. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37°C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 9,11 D16S539: 11,14 D5S818: 11,12 D7S820: 9,12 THO1: 7,10 TPOX: 8 vWA: 15,18 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | S Rasheed |
| Deposited As： | Homo sapiens |
| References： | Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628 Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805 Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 |