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微信小章| 产品名称： | HT-2 clone A5E |
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| 商品货号： | TS211810 |
| Organism： | Mus musculus, mouse |
| Tissue： | spleen |
| Cell Type： | IL-2 dependent T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | C57BL |
| Applications： | The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay. The cells are commonly used for characterization of lymphokines and cytokines. |
| Derivation： | The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of C57BL spleen cells activated by sheep erythrocytes in the presence of IL-2.
This cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at ATCC and by the NIST Mouse STR Consortium using mouse STR technology has shown that the STR profile has more alleles in common with a C57BL mouse strain rather than BALB/c.
Reference: https://www.ncbi.nlm.nih.gov/pubmed/31220119 |
| Antigen Expression： | Thy-1 |
| Receptor Expression： | interleukin 2 (IL-2) |
| Comments： | The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.
In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).
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| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100-200 IU/ml IL-2, 90%; fetal bovine serum, 10%
Alternatively, IL-2 in the form of Rat T-STIM factor with Con A (available from Becton Dickinson, Cat.# 354115) may be used at 10% by volume.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 3.5 x 10 4 viable cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JF Weaver, A Creasey |
| Deposited As： | Mus musculus |
| Year of Origin： | 1979 |
| References： | Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804 Ho SN, et al. Differential bioassay of interleukin-2 and interleukin-4. J. Immunol. Methods 98: 99-104, 1987. PubMed: 3494085 Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412 Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524 . . J. Biol. Response Modif. 4: 96-109, 1985. Kupper T, et al. Autocrine growth of T cells independent of interleukin 2: identification of interleukin 4 (IL 4, BSF-1) as an autocrine growth factor for a cloned antigen-specific helper T cell. J. Immunol. 138: 4280-4287, 1987. PubMed: 2953803 Woods A, et al. Granulocyte-macrophage colony stimulating factor produced by cloned L3T4a+, class II-restricted T cells induces HT-2 cells to proliferate. J. Immunol. 138: 4293-4297, 1987. PubMed: 2953805 Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328 |