微信小陈
微信小章| 产品名称： | HT-3 |
|---|---|
| 商品货号： | TS211811 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix; Derived from metastic site: lymph node, carcinoma |
| Cell Type： | cervical carcinoma |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cervical carcinoma |
| Age： | 58 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P147) hypotriploid to hypertriploid (+A, +B, +C, +D, +F, -E). Modal number = 65 to 66 with abnormalities including dicentrics, acrocentric fragments, minutes, translocations, rings, secondary constrictions, breaks, pulverizations and large subtelocentric and large submetacentric markers. |
| Clinical Data： | 58 years Caucasian female |
| Oncogene： | p53 +; pRB + |
| Genes Expressed： | blood type A; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III); also forms tumors in steroid treated hamsters |
| Comments： | Ultrastructural features include many microvilli, prominent nucleoli, sparse RER, well developed Golgi.
The retinoblastoma protein (pRB) is present but abnormal in size.
P53 expression is elevated and there is a point mutation at codon 245 resulting in a Gly -> Val substitution
The line is negative for human papillomavirus DNA and RNA. No virus particles were observed. A mycoplasma contamination was detected and eliminated in 1965.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 12,13 D5S818: 10,13 D7S820: 8,10 THO1: 6,7 TPOX: 8 vWA: 15,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 |