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微信小章| 产品名称： | HT-144 |
|---|---|
| 商品货号： | TS211813 |
| Organism： | Homo sapiens, human |
| Tissue： | malignant melanoma; derived from metastatic site: subcutaneous tissue |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 29 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P15) hypotriploid to hypertriploid (+A, +B, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, minutes, secondary constrictions, breaks, large submetacentric, subtelocentric and minute markers |
| Derivation： | This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh. |
| Clinical Data： | 29 years Caucasian male |
| Antigen Expression： | Blood Type O; Rh+; HLA A1, Aw24, B13, B15, Cw3, DRw4, DRw7 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms anaplastic malignant tumor consistent with melanoma; tumors also form in steroid treated hamsters |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio:xa0A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal:xa02 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 12,13 D5S818: 11,13 D7S820: 11 THO1: 6,9 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887 |