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| 产品名称： | HuTu 80 |
|---|---|
| 商品货号： | TS211817 |
| Organism： | Homo sapiens, human |
| Tissue： | duodenum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 53 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46; range = 42 to 48. This is a pseudodiploid human cell line. The modal chromosome number is 46 occurring at 68% and polyploidy at 0.4%. Three marker chromosomes were detected in all metaphases examined. Two markers that appeared to be the product of the balanced translocation between N1 and N17. The third marker is ?inv(13). No other common markers were detected. Normal N1, N13, and N17 had single-copy each. Y chromosome is present. |
| Clinical Data： | 53 years Caucasian male |
| Antigen Expression： | Antigen expression: Blood Type B; Rh+ |
| Receptor Expression： | Receptor expression: bombesin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms well differentiated papillary adenocarcinoma, (grade I) |
| Comments： | The cells express receptors for bombesin at up to 6000 sites per cell. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 8,11 D16S539: 10,11 D5S818: 12,13 D7S820: 9,11 THO1: 7 TPOX: 9,11 vWA: 16,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | V Larson |
| Deposited As： | Homo sapiens |