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| 产品名称： | HT 29/36 SKI 294/HT 29/36 |
|---|---|
| 商品货号： | TS211823 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cancer, adenocarcinoma |
| Applications： | The antibody reacts with human colon adenocarcinoma cells. This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human colon adenocarcinoma |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human colon adenocarcinoma |
| Comments： | Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38). The antibody reacts with human colon adenocarcinoma cells. It does not react with frozen sections of normal adult tissues. This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers. |
| Complete Growth Medium： | RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Isotype： | IgG3 |
| Name of Depositor： | Sloan-Kettering Institute for Cancer Research |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. US Patent 4,579,827 dated Apr 1 1986 |