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| 产品名称： | HT-1197 |
|---|---|
| 商品货号： | TS211827 |
| Organism： | Homo sapiens, human |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 44 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | 44 years Caucasian male |
| Genes Expressed： | fibrinolytic activity |
| Cellular Products： | fibrinolytic activity |
| Tumorigenic： | Yes |
| Effects： | Yes, in mice and hamsters |
| Comments： | The cells will also grow in soft agar. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | S Rasheed |
| Deposited As： | Homo sapiens |
| References： | Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628 |