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| 产品名称： | Hs 97.Fs |
|---|---|
| 商品货号： | TS211832 |
| Organism： | Homo sapiens, human |
| Tissue： | skin; foreskin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | male |
| Ethnicity： | American Indian |
| Applications： | This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46 |
| Derivation： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Clinical Data： | male American Indian newborn |
| Comments： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10% |
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days |
| Cryopreservation： | Freeze medium: growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Passage History： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |