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微信小章| 产品名称： | HS-5 |
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| 商品货号： | TS211835 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow/stroma |
| Cell Type： | HPV-16 E6/E7 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 30 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.
The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | Long term bone marrow cells were transformed with the amphotropic retrovirus vector LXSN16E6E7 in the presence of polybrene. Twenty-seven immortalized clones designated HS-1 to HS-27 were isolated. |
| Clinical Data： | 30 years Caucasian male |
| Genes Expressed： | granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), macrophage-CSF (M-CSF), Kit ligand (KL), macrophage-inhibitory protein-1 alpha, interleukin-1 alpha (IL-1alpha), IL-1beta, IL-1RA, IL-6, IL-8, IL-11, and leukemia inhibitory factor (LIF) |
| Comments： | One of these cell lines HS-27A (ATCC CRL-2496) has been deposited in the ATCCs general collection. One cell line (HS-5) has been deposited in the Patent Depository (CRL-11882). HS-5 supports proliferation of hematopoietic progenitor cells when co-cultured in serum-deprived media with no exogenous factors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:9 is recommended
Medium Renewal: Everh 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | Fred Hutchinson Cancer Res. Cntr. |
| Deposited As： | human |
| U.S. Patent Number： | |
| References： | Roecklein BA, Torok-Storb B. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Blood 85: 997-1005, 1995. PubMed: 7849321 Torok-Storb B, et al. Human marrow stromal cell lines which sustain hematopoieses. US Patent 5,879,940 dated Mar 9 1999 |