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| 产品名称： | Hs 895.Sk |
|---|---|
| 商品货号： | TS211856 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast,Melanoma |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 48 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The two lines provide tumor and normal counterparts for comparative in vitro studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46; range = 32 to 46 |
| Derivation： | This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma. |
| Comments： | Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |