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| 产品名称： | Hs 746T |
|---|---|
| 商品货号： | TS211883 |
| Organism： | Homo sapiens, human |
| Tissue： | stomach; derived from metastatic site: left leg |
| Cell Type： | Epithelial,fibroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | gastric carcinoma |
| Age： | 74 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypertriploid human cell line having the modal chromosome number of 77 occurring in 46% of cells. Cells with higher ploidies were found at 16.6%. The karyotype of this cell line is very complex. Between 45% and 55% of chromosomes in each cell had structural aberrations, including: der(4)t(1;4) (p13.1;q35); t(15q22q); del(2) (p13); der(7)t(7;?) (p22;?) 7p+; t(7q22q?) and others. The t(15q22q), del(2), and t(7q22q?) (and several others) had two copies in most cells. There were two types of der(7): one was the 7p+ described above; the other, 7p++, was distinctly longer than N1. There were more than ten markers that were present in some cells only. One DM-like chromosome was found in most cells. Normal N7 and N21 were absent. One normal X and one or two normal Y chromosomes were detected. |
| Clinical Data： | 74 years adult Caucasian male |
| Tumorigenic： | Yes |
| Effects： | Yes, in immunosuppressed mice |
| Comments： | In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10 D16S539: 11 D5S818: 11,13 D7S820: 8 THO1: 9 TPOX: 8,10 vWA: 15,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | RB Owens |
| Deposited As： | Homo sapiens |
| Passage History： | In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts. |
| Year of Origin： | 1973 |
| References： | Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258 The cells form carcinomas. |