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微信小章| 产品名称： | Hs 683 |
|---|---|
| 商品货号： | TS211901 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | glioma |
| Age： | 76 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells. |
| Derivation： | Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. |
| Clinical Data： | 76 years
Caucasian
male |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | Microvilli, but no desmosomes were observed. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 THO1: 6,8 TPOX: 8,11 vWA: 18,20 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | RB Owens |
| Deposited As： | Homo sapiens |
| References： | Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406 |
| Cross References： | Nucleotide (GenBank) : M84526 Human adipsin/complement factor D mRNA, complete cds. |