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| 产品名称： | Hs 274.T |
|---|---|
| 商品货号： | TS211951 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 66 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Karyotype： | modal number = 46; range = 45 to 47 |
| Images： |  |
| Derivation： | Hs 274.T cell line was derived from an adenocarcinoma breast mammary gland of a 66 year old Caucasian female. |
| Clinical Data： | female Caucasian 66 years |
| Comments： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 11, 12 D13S317: 11 D16S539: 11 D5S818: 12, 13 D7S820: 11 THO1: 9, 9.3 TPOX: 8, 10 vWA: 14, 17 |
| Passage History： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |