微信小陈
微信小章| 产品名称： | HPAF-II |
|---|---|
| 商品货号： | TS211972 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 44 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes.xad |
| Clinical Data： | Caucasian
44 years
male |
| Antigen Expression： | Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3 |
| Genes Expressed： | mucin |
| Cellular Products： | mucin |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells form well differentiated adenocarcinomas in athymic mice which resemble the original tumor |
| Comments： | HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells. The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture. xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 12 D16S539: 11,13 D5S818: 11,13 D7S820: 10,13 F13A01: 5,17 F13B: 8,10 FESFPS: 11,12 LPL: 10 THO1: 9 TPOX: 8 vWA: 17 |
| Name of Depositor： | RS Metzgar |
| Deposited As： | Homo sapiens |
| References： | Monoclonal antibodies in cancer. Clifton, NJ: Humana Press; 1985. Monoclonal antibodies and cancer therapy. New York: Liss; 1985. Immunity to cancer II. New York: Liss; 1989. Metzgar RS, et al. Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res. 42: 601-608, 1982. PubMed: 7034925 Kim YW, et al. Characterization of clones of a human pancreatic adenocarcinoma cell line representing different stages of differentiation. Pancreas 4: 353-362, 1989. PubMed: 2734279 Mullins TD, et al. Ultrastructural differentiation of sodium butyrate-treated human pancreatic adenocarcinoma cell lines. Pancreas 6: 578-587, 1991. PubMed: 1946315 Worlock AJ, et al. Radiolocalization of human pancreatic tumors in athymic mice by monoclonal antibody DU-PAN 1. Cancer Res. 50: 7246-7251, 1990. PubMed: 2224857 |