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微信小章| 产品名称： | HPAC |
|---|---|
| 商品货号： | TS211974 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 64 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 61 |
| Derivation： | HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin. |
| Clinical Data： | 64 years
female
Caucasian |
| Receptor Expression： | epidermal growth factor (EGF), expressed glucocorticoid, expressed epidermal growth factor (EGF); glucocorticoid |
| Genes Expressed： | HPAC cells are positive for keratin and negative for vimentin and chromogranin A. |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose. |
| Comments： | HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha). Cell growth is suppressed by dexamethasone and other glucocorticoids. Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.
In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells. HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor. Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6, every 6 to 8 days.xa0 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature:xa037°C Atmosphere:xa0Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,10 D5S818: 12 D7S820: 10,12 THO1: 9.3 TPOX: 10,11 vWA: 15,17 |
| Population Doubling Time： | 41 hrs |
| Name of Depositor： | WR Gower |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1985 |
| References： | Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994. |