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微信小章| 产品名称： | HP6045 |
|---|---|
| 商品货号： | TS211987 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | mixed suspension-adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with human IgG, gamma heavy chain, F(ab) and Fab fragments but not with Fc fragment or rabbit IgG. The antibody from this cell line was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified human myeloma protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human IgG (Fd, F(ab)2, Fab) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human IgG (Fd, F(ab)2, Fab) |
| Comments： | Animals were immunized with purified human myeloma protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human IgG, gamma heavy chain, F(ab) and Fab fragments but not with Fc fragment or rabbit IgG. The reactivity is comparable to that of conventional rabbit anti human Fd reagents in radioimmunoassays for rheumatoid factor (RF). The antibody from this cell line was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, supplemented with:10 mM HEPES , 0.05 mM 2-mercaptoethanol and 10% fetal bovine serum
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| Subculturing： | Scrape attached cells. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2a |
| Name of Depositor： | CB Reimer |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923 McDougal JS, et al. Immunoassay for IgG rheumatoid factor with a murine monoclonal anti-Fd antibody. J. Lab. Clin. Med. 106: 80-87, 1985. PubMed: 4009026 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |