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| 产品名称： | HO-22-1 |
|---|---|
| 商品货号： | TS211988 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Thy-1.1 antigen |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Thy-1.1 antigen |
| Comments： | Animals were immunized with AKR/J thymocytes. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, with non-essential amino acids, 80%; fetal bovine serum, 20%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 exp4 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | A Marshak-Rothstein |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Marshak-Rothstein A, et al. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J. Immunol. 122: 2491-2497, 1979. PubMed: 87465 |