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| 产品名称： | HO-2.2 |
|---|---|
| 商品货号： | TS211993 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The Lyt-2.2 antigen is present on mouse cytotoxic and suppressor T cells and is equivalent to the Lyt-2b serologic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse T cell antigen); cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse T cell antigen); cytotoxic |
| Comments： | Animals were immunized with C57BL/6 thymocytes. Spleen cells were fused with P3X63Ag8 myeloma cells. The Lyt-2.2 antigen is present on mouse cytotoxic and suppressor T cells and is equivalent to the Lyt-2b serologic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | P Gottlieb |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Gottlieb PD, et al. Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt-3.1 monoclonal antibodies. Immunogenetics 10: 545-555, 1980. Raulet DH, et al. Fractionation of lymphocyte populations with monoclonal antibodies specific for Lyt-2.2 and Lyt-3.1. J. Immunol. 125: 1136-1143, 1980. PubMed: 6447723 |