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购买须知
| 产品名称： | HMCB Human Melanoma Cell Bowes |
|---|---|
| 商品货号： | TS211994 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | melanoma |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed： | human tissue plasminogen activator |
| Cellular Products： | human tissue plasminogen activator |
| Comments： | The cells originally tested positive for mycoplasma. After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: culture medium 95%; DMSO 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | Genentech, Inc. |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： |