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| 产品名称： | HOPC 1F/12 |
|---|---|
| 商品货号： | TS211995 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma. The cells are resistant to cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma. |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma. The cells are resistant to cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a; lambda 1 light chain |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 Weigert MG, et al. Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159 |