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微信小章| 产品名称： | HIT-T15 |
|---|---|
| 商品货号： | TS212001 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | pancreas/islet of Langerhans |
| Cell Type： | beta cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40. |
| Receptor Expression： | glucagon; somatostatin; glucocorticoid |
| Genes Expressed： | insulin |
| Comments： | The cells secrete up to 24 ng of insulin per 106 cells per 24 hours (passage 60).
Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.
Insulin synthesis decreases with length of time in culture. |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Interval: An inoculum of approximately 1.3xa0x 105 viable cells perxa0cm2 is recommended.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | R Santerre |
| Deposited As： | Mesocricetus auratus |
| References： | Lord JM, Ashcroft SJ. Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells. Biochem. J. 219: 547-551, 1984. PubMed: 6234883 Santerre RF, et al. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc. Natl. Acad. Sci. USA 78: 4339-4343, 1981. PubMed: 6270673 Aguilar-Bryan L, et al. Co-expression of sulfonylurea receptors and KATP channels in hamster insulinoma tumor (HIT) cells. Evidence for direct association of the receptor with the channel. J. Biol. Chem. 267: 14934-14940, 1992. PubMed: 1634534 |
| Cross References： | Nucleotide (GenBank) : U62810 Mesocricetus auratus potassium channel Kv8.1 mRNA, complete cds. |