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| 产品名称： | HH |
|---|---|
| 商品货号： | TS212016 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cutaneous T cell lymphoma |
| Age： | 61 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis. |
| Clinical Data： | 61 years adult The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis. Caucasian male |
| Antigen Expression： | CD2; Homo sapiens, expressed CD3; Homo sapiens, expressed CD4; Homo sapiens, expressed CD8; Homo sapiens; negative CD25; Homo sapiens; negative CD30; Homo sapiens, expressed HLA-DR; Homo sapiens, expressed |
| Receptor Expression： | interleukin 2 (IL-2) beta chain, expressed |
| HeLa Markers： | N |
| Comments： | Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex. A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 12 D5S818: 11,13 D7S820: 10 THO1: 9 TPOX: 8,11 vWA: 15,16 |
| Name of Depositor： | G Starkebaum |
| Deposited As： | Homo sapiens |
| References： | Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969 HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy. |