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| 产品名称： | HEK001 |
|---|---|
| 商品货号： | TS212020 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 65 year old |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The HEK001(human epidermal keratinocyte 001) cell line was established in 1996. Cells from the scalp of a 65 year old male were transfected with plasmid p1321(a generous gift from Dr. Karl Munger, Harvard Medical School). Plasmid p1321 contains human papillomavirus (HPV) 16 E6 and E7 genes. |
| Clinical Data： | male
65 years |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Comments： | HEK001 cells express keratin 14 but not keratin 10 suggesting that they are proliferating basal-type keratinocytes. |
| Complete Growth Medium： | Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 5 ng/ml human recombinant EGF and 2mM L-glutamine (without bovine pituitary extract and without serum)
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Add fresh medium twice a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.xa0xa0
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| Cryopreservation： | Freeze medium: Dulbeccos Modified Eagles Medium, 70%; fetal bovine serum, 20%; DMSO, 10%. Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11 D13S317: 8 D16S539: 12,13 D5S818: 10,13 D7S820: 12,13 THO1: 6,9 TPOX: 8,9 vWA: 14 |
| Name of Depositor： | PB Sugerman, M Bigby |
| Deposited As： | human |
| Year of Origin： | 1996 |
| References： | Sugerman PB, Bigby M. Preliminary functional analysis of human epidermal T cells. Arch. Dermatol. Res. 292: 9-15, 2000. PubMed: 10664009 |