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微信小章| 产品名称： | HEL 92.1.7 |
|---|---|
| 商品货号： | TS212022 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | erythroblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | erythroleukemia |
| Age： | 30 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | 30 years Caucasian male |
| Antigen Expression： | Antigen expression: HLA A3, Aw32, Bw35; Ia+ |
| Genes Expressed： | hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin |
| Comments： | These cells differentiate spontaneously into erythroblast-like cells. Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 7 THO1: 7 TPOX: 11 vWA: 14,17 |
| Name of Depositor： | PJ Martin |
| Deposited As： | Homo sapiens |
| References： | Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. PubMed: 6309287 Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. PubMed: 6177045 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Gan W, Rhoads RE. Internal initiation of translation directed by the 5-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 Riewald M, et al. Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. PubMed: 8636153 |