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| 产品名称： | Hepa 1-6 Hepa1-6 |
|---|---|
| 商品货号： | TS212025 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Strain： | C57L |
| Applications： | This line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. |
| Genes Expressed： | albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase |
| Cellular Products： | albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Note: The cells can also be propagated in a serum free medium consisting of 3 parts Dulbeccos modified Eagles medium, 1 part Waymouths MAB 87/3 medium and 30 nM selenium. |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | GJ Darlington |
| Deposited As： | Mus musculus |
| References： | Darlington GJ. Liver cell lines. Methods Enzymol. 151: 19-38, 1987. PubMed: 3431441 Darlington GJ, et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl. Cancer Inst. 64: 809-819, 1980. PubMed: 6102619 |