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微信小章| 产品名称： | Hepa-1c1c7 |
|---|---|
| 商品货号： | TS212026 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Strain： | C57L |
| Applications： | This and mutant derivatives of it are useful for studying the aryl hydrocarbon (Ah) receptor and P450IA1 regulation. This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. |
| Receptor Expression： | aryl hydrocarbon (Ah) |
| Genes Expressed： | cytochrome P450IA1 |
| Cellular Products： | cytochrome P450IA1 |
| Comments： | The cells are highly inducible for cytochrome P450IA1. |
| Complete Growth Medium： | Alpha minimum essential medium without nucleosides, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 18 hrs |
| Name of Depositor： | O Hankinson |
| Deposited As： | Mus musculus |
| References： | Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668 Hankinson O, et al. Genetic and molecular analysis of the Ah receptor and of Cyp1a1 gene expression. Biochimie 73: 61-66, 1991. PubMed: 1851644 Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390 |