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微信小章| 产品名称： | HEPM |
|---|---|
| 商品货号： | TS212027 |
| Organism： | Homo sapiens, human |
| Tissue： | palatal mesenchyme |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human diploid cell line with the 46,XX karyotype. The modal chromosome number was 46, occurring in 76% of cells. The rate of cells with polyploidies was 4.2%. No consistent chromosome aberrations were detected. |
| Derivation： | HEPM was derived from mesenchymal cells from the human embryo by M Macy. |
| Clinical Data： | female
fetus |
| Receptor Expression： | epidermal growth factor (EGF) |
| Comments： | The cells are highly responsive to epidermal growth factor (EGF). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 8,12 D16S539: 11,12 D5S818: 11,13 D7S820: 8,10 THO1: 6,9.3 TPOX: 8,11 vWA: 17,18 |
| Name of Depositor： | M Macy |
| Deposited As： | Homo sapiens |
| Year of Origin： | July, 1979 |
| References： | Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936 Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523 Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981. Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630 |