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微信小章| 产品名称： | HCN-2 |
|---|---|
| 商品货号： | TS212028 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Cell Type： | cortical neuron |
| Product Format： | frozen |
| Morphology： | neuronal |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | encephalitis |
| Age： | 7 years |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The HCN-2 cell line was derived from cortical tissue removed from a patient undergoing hemispherectomy for intractable seizures associated with Rasmussens encephalitis. |
| Clinical Data： | female
7 years |
| Genes Expressed： | tubulin; neurofilament protein; somatostatin; cholecystokinin-8 |
| Cellular Products： | tubulin; neurofilament protein; somatostatin; cholecystokinin-8 |
| Comments： | HCN-2 cells can be induced to differentiate when cultured with a mixture of nerve growth factor (NGF), dibutyryl cyclic adenosine monophosphate (cAMP) and 1-isobutyl-3-methylxanthine (IBMX). Differentiation is accompanied by mature morphology and slowing of growth (doubling time greater than 120 hours). The growth rate of HCN-2 cells is stimulated by treatment with phorbol esters. A similar line (HCN-1A) is available as ATCC CRL-10442. The cells stain positively for a number of neuronal markers including neurofilament protein, neuron specific enolase (NSE). They are also positive for tubulin, vimentin, somatostatin (SST), glutamate, gamma aminobutyric acid (GABA), cholecystokinin - 8 (CCK-8) and vasoactive intestinal peptide (VIP). The cells are negative for glial fibrillary acidic protein (GFAP) and myelin basis protein (MBP).
CRL-10742 has been shown to senesce at approximately passage 21. Current distribution stocks are prepared with only 4-5 Population Doublings (PDLs) remaining under recommended culture conditions after cryopreservation. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
NOTE: This cell line is extremely slow growing. May not be able to subculture for 12 to 14 days after recovery. Cultures do not reach 100% confluence (about 80-90%). A change in the fetal bovine serum may help increase the growth rate. Subculture at no higher than a 1:3 ratio every 10 to 12 days when cultures are about 90% confluent.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,15 D13S317: 11,12 D16S539: 8,11 D5S818: 13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8 vWA: 14,20 |
| Name of Depositor： | Johns Hopkins University |
| U.S. Patent Number： | |
| Passage History： | CRL-10742 has been shown to senesce at approximately passage 21. Current distribution stocks are prepared with only 4-5 Population Doublings (PDLs) remaining under recommended culture conditions after cryopreservation. |
| References： | Ronnett GV, et al. Human neuronal cell line. US Patent 5,196,315 dated Mar 23 1993 |