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微信小章| 产品名称： | HD168 |
|---|---|
| 商品货号： | TS212029 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leukemia |
| Applications： | The antibody is useful in studies of mouse class I antigens and T cell receptors. The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen. |
| Derivation： | A rat was immunized with the TL+ mouse leukemia ASL1 (a spontaneous leukemia that developed in a strain A mouse). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen. The antibody is useful in studies of mouse class I antigens and T cell receptors. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse TL antigen |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse TL antigen |
| Tumorigenic： | Yes |
| Effects： | Yes, in pristane primed nu/nu mice |
| Comments： | A rat was immunized with the TL+ mouse leukemia ASL1 (a spontaneous leukemia that developed in a strain A mouse). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen. The antibody is useful in studies of mouse class I antigens and T cell receptors. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 0.016 mM thymidine, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | ES Stockert |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Obata Y, et al. Structural analysis of TL genes of the mouse. Proc. Natl. Acad. Sci. USA 82: 5475-5479, 1985. PubMed: 2410925 Obata Y, et al. Influence of 5 flanking sequences on TL and H-2 expression in transfected L cells. Proc. Natl. Acad. Sci. USA 85: 3541-3545, 1988. PubMed: 3368465 Hamasima N, et al. Expression of TL, H-2, and chimeric H-2/TL genes in transgenic mice: abnormal thymic differentiation and T-cell lymphomas in a TL transgenic strain. Proc. Natl. Acad. Sci. USA 86: 7995-7999, 1989. PubMed: 2813374 Morita A, et al. TL antigen as a transplantation antigen recognized by TL-restricted cytotoxic T cells. J. Exp. Med. 179: 777-784, 1994. PubMed: 8113675 |