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| 产品名称： | HCC202 |
|---|---|
| 商品货号： | TS212038 |
| Organism： | Homo sapiens, human |
| Tissue： | breast; mammary gland/duct |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Culture Properties： | adherent, The line grows as attached medium-sized epithelial cells with some floating cells. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIIA, grade 3, primary ductal carcinoma |
| Age： | 82 years |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Karyotype： | polyploid |
| Derivation： | The HCC202 cell line was initiated from a primary ductal carcinoma on September 5, 1992, and took 41 months to establish. |
| Clinical Data： | 82 years Caucasian, White female |
| Receptor Expression： | estrogen receptor, negative progesterone receptor, negative |
| Oncogene： | her2/neu +, p53 - |
| Genes Expressed： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Comments： | The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 19 lymph nodes.
The cells are poorly differentiated. The cells are positive for expression of Her2-neu, but are negative for expression of p53. HCC202 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -) and progesterone receptor (PR -).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:2 to 1:3 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 8,9 D16S539: 13 D5S818: 11,13 D7S820: 8,12 THO1: 6 TPOX: 8,9 vWA: 16 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Deposited As： | Homo sapiens |
| Year of Origin： | September 5, 1992 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |