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| 产品名称： | HCC2157 |
|---|---|
| 商品货号： | TS212043 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIIA, grade 2,primary ductal carcinoma |
| Age： | 48 years adult |
| Gender： | female |
| Ethnicity： | Black |
| Applications： | HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). The cells are positive for expression of Her2-neu and for expression of p53. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% |
| Images： |  |
| Derivation： | This cell line was initiated on 3/4/96 and took 8 months to establish.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341. |
| Clinical Data： | Black
female
48 years
|
| Receptor Expression： | estrogen receptor, negative progesterone receptor, positive |
| Oncogene： | her2/neu +, p53 + |
| Genes Expressed： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Comments： | The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341. |
| Complete Growth Medium： | RPMI-1640 medium, 95%; fetal bovine serum, 5% |
| Subculturing： | Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 13 D7S820: 10,12 THO1: 8,9 TPOX: 9,11 vWA: 14,18 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | 1996 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |