mammary gland; breast/duct

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells.

The cells are poorly differentiated.

The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.

The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.

The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.

49 years

Black

female

progesterone receptor, not expressed

Epithelial glycoprotein 2 EGP2; cytokeratin 19,her2/neu -, p53 + (overexpressed),The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.,The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.

Epithelial glycoprotein 2 EGP2; cytokeratin 19

The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.

The cells are poorly differentiated.

The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes.

The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.

HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. An independent study, by ATCC Scientists, is underway to assess the estrogen receptor status; results of this study will be published online upon completion.

Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product.
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Amelogenin:X

CSF1PO:10,14

D13S317:12

D16S539:9,13

D5S818:12,13

D7S820:10,11

THO1:9

TPOX:10

vWA: 13,15