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| 产品名称： | HCC1500 |
|---|---|
| 商品货号： | TS212052 |
| Organism： | Homo sapiens, human |
| Tissue： | breast; mammary gland/duct |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIB, grade 2, primary ductal carcinoma |
| Age： | 32 years |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near diploid |
| Derivation： | This cell line was initiated on 2/8/95 from a patient with a significant family history of early-onset colon cancer as well as a sister with breast cancer. The cell line took 14 months to establish. |
| Clinical Data： | 32 years
Black
female
|
| Receptor Expression： | estrogen receptor progesterone receptor |
| Oncogene： | her2/neu -, p53 + |
| Genes Expressed： | Epithelial glycoprotein 2 (EGP2),cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 (EGP2) cytokeratin 19 |
| Comments： | The tumor was classified as TNM stage IIB, grade 2, invasive ductal carcinoma with 4 out of 24 lymph node metastasis.
The cells are poorly differentiated.
The cells are negative for expression of Her2-neu but positive for expression of p53.
HCC1500 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are positive for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
HCC1500 contains a homozygous deletion at 3p21.3. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:2 to 1:3 Medium Renewal: Everyxa03 toxa05 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 9,10 D5S818: 11,13 D7S820: 11 THO1: 9 TPOX: 8 vWA: 13,16 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | February 8, 1995 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 Sekido Y, et al. Cloning of a breast cancer homozygous deletion junction narrows the region of search for a 3p21.3 tumor suppressor gene. Oncogene 16: 3151-3157, 1998. PubMed: 9671394 |