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| 产品名称： | HCC1599 |
|---|---|
| 商品货号： | TS212054 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct |
| Cell Type： | epithelial, lymphoblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIIA, grade 3, primary ductal carcinoma |
| Age： | 44 years adult |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Applications： | HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). The cells are negative for expression of Her2-neu and for expression of p53. The cells are poorly differentiated. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | multiploid. The cell population has several ploidy indices. |
| Images： |  |
| Derivation： | The HCC1599 breast cancer cell line was initiated on March 28, 1995, and took 10 months to establish. |
| Clinical Data： | 44 years
An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.
Caucasian, White
female |
| Receptor Expression： | estrogen receptor, negative progesterone receptor, negative |
| Oncogene： | her2/neu -, p53 - |
| Genes Expressed： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Comments： | The tumor was classified as a TNM stage IIIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available. The cells are poorly differentiated. The cells are negative for expression of Her2-neu and for expression of p53. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19. An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12,13 D5S818: 12 D7S820: 10,11 THO1: 9.3 TPOX: 8 vWA: 17 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | March 28, 1998 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |