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| 产品名称： | HCC1954 |
|---|---|
| 商品货号： | TS212056 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | large epithelial cells with occasional vacuoles |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIA, grade 3, ductal carcinoma |
| Age： | 61 years adult |
| Gender： | female |
| Ethnicity： | East Indian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. The HCC1954 is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish. |
| Clinical Data： | 61 years adult East Indian female |
| Receptor Expression： | estrogen receptor, not expressed progesterone receptor, not expressed |
| Oncogene： | her2/neu + (overexpressed) |
| Genes Expressed： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 EGP2; cytokeratin 19 |
| Comments： | HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
Her2/neu is overexpressed in the ELISA assay.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:8 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 8,9 D16S539: 9,11 D5S818: 11 D7S820: 10,11 THO1: 6,7 TPOX: 8,9 vWA: 18,19 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | October 30, 1995 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |