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微信小章| 产品名称： | HCC1187 |
|---|---|
| 商品货号： | TS212063 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IIA, grade 3, primary ductal carcinoma |
| Age： | 41 years adult |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | multiploid; cell population has several ploidy indices |
| Derivation： | This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish. |
| Clinical Data： | 41 years
Caucasian
female
|
| Receptor Expression： | progesterone receptor, negative |
| Oncogene： | her2/neu -, p53 + |
| Cellular Products： | Epithelial glycoprotein 2 (EGP2) cytokeratin 19 |
| Comments： | The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.
The cells are poorly differentiated.
An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323.
HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of Her2-neu; they overexpress p53.
The cells are negative for expression of progesterone receptor (PR).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. Cultures can be maintained by the addition of fresh medium to the flask, or centrifuging and removing the spent medium, resuspending the cell pellet in fresh medium and returning the resuspended cells to the flask. To subculture the attached cells, remove and reserve the suspended cells (recover the suspended cells by centrifugation if desired). Rinse the monolayer with fresh 0.25% trypsin-0.53 mM EDTA, neutralize with an equal volume complete medium and centrifuge. Remove supernatant and resuspend cell pellet with complete medium and pool into reserved suspended cells. Add appropriate aliquots of the pooled cell suspension to new culture vessels. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 10 D5S818: 12 D7S820: 8,11 THO1: 6 TPOX: 8 vWA: 19 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |