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微信小章| 产品名称： | HCC1395 |
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| 商品货号： | TS212065 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct |
| Cell Type： | Epithelial, lymphoblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage I, grade 3, primary ductal carcinoma |
| Age： | 43 years adult |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | polyploid |
| Derivation： | This cell line was initiated on 12/14/94 from a patient with a family history of cancer (patients mother had breast cancer).xa0The cell line took 14 months to establish. |
| Clinical Data： | female
43 years
Caucasian, White
An EBV transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325. |
| Receptor Expression： | estrogen receptor, negative according to depositor progesterone receptor, negative |
| Oncogene： | her2/neu -, p53 + |
| Genes Expressed： | Epithelial glycoprotein 2 (EGP2),cytokeratin 19 |
| Cellular Products： | Epithelial glycoprotein 2 (EGP2) cytokeratin 19 |
| Comments： | The tumor was classified as TNM stage I, grade 3, invasive ductal carcinoma with 0 out of 34 lymph node metastasis.
The cells are poorly differentiated and vacuolated.xa0 The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -). An EBV transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:2 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 9 D16S539: 11 D5S818: 11 D7S820: 10,12 THO1: 9.3 TPOX: 11 vWA: 15 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | December 14, 1994 |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |