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微信小章| 产品名称： | HCC1428 |
|---|---|
| 商品货号： | TS212066 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast; derived from metastatic site: adenocarcinoma and pleural effusion |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent; large epithelial cells with occasional vacuole formation |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | TNM stage IV, grade 4, adenocarcinoma |
| Age： | 49 years |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | polyploid |
| Derivation： | HCC1428 was derived from a female who harbored a homozygous deletion in the FHIT gene. This cell line was initiated on 11/4/95 and took 15 months to establish. |
| Clinical Data： | female
Caucasian, White
49 years
There is a family history of breast cancer (maternal grandmother). |
| Oncogene： | her2/neu -, p53 - |
| Genes Expressed： | HCC1428 cells are positive for the expression of Epithelial glycoprotein 2 EGP2; cytokeratin 19 The cells are negative for expression of Her2-neu and for expression of p53. |
| Comments： | An EBV transformed lymphoblastoid cell line HCC1428 BL from the same patient is available as ATCC CRL-2328. The homozygous deletion in the FHIT gene is retained in the lymphoblastoid line. The FHIT gene, which spans the FRA3B fragile site at chromosome 3p14.2, is a candidate tumor suppressor gene in breast and other cancers. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:8 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | CSF1PO: 10 D13S317: 12 D16S539: 12,8 D5S818: 11,12 D7S820: 9 THO1: 8 TPOX: 8 vWA: 17 |
| Name of Depositor： | AF Gazdar, AK Virmani |
| Year of Origin： | November 4, 1995 |
| References： | Ahmadian M, et al. Analysis of the FHIT gene and FRA3B region in sporadic breast cancer, preneoplastic lesions, and familial breast cancer probands. Cancer Res. 57: 3664-3668, 1997. PubMed: 9288768 Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 |